Isolation and characterization of microsatellite loci from Psidium guajava L
نویسندگان
چکیده
A (GA) n and (GT) n microsatellite-enriched library was constructed and 23 nuclear simple sequence repeat (SSR) loci were characterized in the guava species ( Psidium guajava L.). All SSR loci were found to be polymorphic after screening for diversity in different cultivars, and across-taxa amplification tests showed the potential transferability of most SSR markers in three other Psidium species. First to be published for P. guajava , this new SSR resource will be a powerful tool for genetic studies of guava, including cultivars identification and linkage mapping, as well as potentially for interspecific genetic studies within the genus Psidium .
منابع مشابه
Microsatellite Isolation and Characterization in Pomegranate (Punica granatum L.)
Development of microsatellite markers has been an increasing trend in crop genetic studies because oftheir applicability in breeding programs. Here we report the development of inter simple sequencerepeat (SSRs) in pomegranate (Punica granatum L.) using an enrichment method that makes use of magneticbeads. Enriched genomic libraries with AG and ATG microsatellite motifs were c...
متن کاملEvaluation of Antimicrobial and Antioxidant Activities of Psidium guajava L growing in Libya
The aim of this study was to evaluate quantitatively the antimicrobial and antioxidant potentials of the leaves extracts of Guava (Psidium guajava Linn.),belonging to the Myrtaceae family. The plant samples were collected from Tajoura eastern part of Tripoli in Libya. Methanol and water were used as solvents in the extraction. The phytochemical screening showed that the ethanolic and aqueous ex...
متن کاملIsolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
متن کاملIsolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
متن کاملEvaluation of Antimicrobial and Antioxidant Activities of Psidium guajava L growing in Libya
The aim of this study was to evaluate quantitatively the antimicrobial and antioxidant potentials of the leaves extracts of Guava (Psidium guajava Linn.),belonging to the Myrtaceae family. The plant samples were collected from Tajoura eastern part of Tripoli in Libya. Methanol and water were used as solvents in the extraction. The phytochemical screening showed that the ethanolic and aqueous ex...
متن کامل